Inaba, K., Murakami, S., Nakagawa, A., Iida, H., Kinjo, M., Ito, K. and Suzuki, M. (2009) Dynamic nature of disulphide bond formation catalysts revealed by crystal structures of DsbB. EMBO J. 28, 779-791.
2008年
Inaba, K., Suzuki, M., Maegawa, K. I., Akiyama, S., Ito, K. and Akiyama, Y. (2008) A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of E. coli. J. Biol. Chem. 283, 35042-35052.
Tsukazaki, T. a), Mori, H. a), Fukai, S., Ishitani, R., Mori, T., Dohmae, N., Perederina, A., Sugita, Y., Vassylyev, D. G., Ito, K. b) and Nureki, O. b) (2008) Conformational transition of Sec machinery inferred from bacterial SecYE structures. Nature 455, 988-991. a) equally contributed, b) corresponding authors
Muto, H. and Ito, K. (2008) Peptidyl-prolyl-tRNA at the ribosomal P-site reacts poorly with puromycin. Biochem. Biophys. Res. Commun. 366, 1043-1047.
Koide, K., Ito, K, and Akiyama, Y. (2008) Substrate recognition and binding by RseP, an Escherichia coli intramembrane protease. J. Biol. Chem. 283, 9562-9570.
2007年
Wang, Y. a), Maegawa, S. a), Akiyama, Y. b) and Ha, Y. b) (2007) The role of L1 loop in the mechanism of rhomboid intramembrane protease GlpG. J. Mol. Biol. 374, 1104-1113. a) equally contributed, b) corresponding authors
Akiyama, Y. and Maegawa, S. (2007) Sequence features of substrates required for cleavage by GlpG, an Escherichia coli rhomboid protease. Mol. Microbiol. 64, 1028-1037.
Maegawa, S., Koide, K., Ito, K., and Akiyama, Y. (2007) The intramembrane active site of GlpG, an Escherichia coli rhomboid protease, is accessible to water and hydrolyzes an extramembrane peptide-bond of substrates. Mol. Microbiol. 64, 435-447.
Koide, K., Maegawa, S., Ito, K., and Akiyama, Y. (2007) Environments of the active site region of RseP, an Escherichia coli RIP protease, assessed by site-directed cysteine alkylation. J. Biol. Chem. 282, 4553-4560.
Shimohata, N., Nagamori, S., Akiyama, Y., Kaback, H.R., and Ito, K. (2007) SecY alterations that impair membrane protein folding and generate a membrane stress. J. Cell Biol. 176, 307-317.
2006年
Vassylyev, D. G., Mori, H., Vassylyeva, M. N., Tsukazaki, T., Kimura, Y., and Ito, K. (2006) Crystal structure of the translocation ATPase SecA from Thermus thermophilus reveals a parallel, head-to-head dimer. J. Mol. Biol. 364, 248-258.
Vassylyeva, M. N., Mori, H., Tsukazaki, T., Yokoyama, S., Tahirov, T. H., Ito, K. and Vassylyev, D. G. (2006) Cloning, expression, purification, crystallization and initial crystallographic analysis of the preprotein translocation ATPase SecA from Thermus thermophilus. Acta Cryst. F62, 909-912.
Tsukazaki, T., Mori, H., Fukai, S., Numata, T., Perederin, A., Adachi, H., Matsumura, H., Takano, K., Murakami, S., Inoue, T., Mori, Y., Sasaki, T., Vassylyev, D., Nureki, O. and Ito, K. (2006) Purification, crystallization and preliminary X-ray diffraction of SecDF, a translocon-associated membrane protein, from Thermus thermophilus. Acta Cryst. F62, 376-380.
Chiba, S., Ito, K. and Akiyama, Y. (2006) The Escherichia coli plasma membrane contains two PHB (prohibitin homology) domain protein complexes of opposite orientations. Mol. Microbiol. 60, 448-457.
Mori, H. and Ito, K. (2006) The long a-helix of SecA is important for the ATPase coupling of translocation. J. Biol. Chem. 281, 36249-36256.
Mori, H. and Ito, K. (2006) Different modes of SecY-SecA interactions revealed by site-directed in vivo photo-crosslinking. Proc. Natl. Acad. Sci. USA. 103, 16159-16164.
2005年
Nakatogawa, H., Murakami, A.、Mori, H. and Ito, K. (2005) SecM facilitates translocase function of SecA by localizing its biosynthesis. Genes Dev. 19, 436-444.
Maegawa, S., Ito, K. and Akiyama, Y. (2005) Proteolytic action of GlpG, a rhomboid protease in the Escherichia coli cytoplasmic membrane. Biochemistry 41, 13543-13552.
Sakoh, M., Ito, K. and Akiyama, Y. (2005) Proteolytic activity of HtpX, a membrane-bound and stress-controlled protease of E. coli. J. Biol. Chem. 280, 33305-33310.
Shimohata, N., Akiyama, Y. and Ito, K. (2005) Peculiar properties of DsbA in its export across the E. coli cytoplasmic membrane. J. Bacteriol. 187, 3997-4004.
2004年
Akiyama, Y., Kanehara, K. and Ito, K. (2004) RseP (YaeL), an E. coli RIP protease, cleaves transmembrane sequences. EMBO J. 23, 4434-4442.
Mori, H., Shimokawa, N., Satoh, Y. and Ito, K. (2004) Mutation analysis of transmembrane regions 3 and 4 of SecY, a central component of protein translocase. J. Bacteriol. 186, 3960-3969.
Saikawa, N., Akiyama, Y. and Ito, K. (2004) FtsH exists as an exceptionally large complex containing HflKC in the plasma membrane of Escherichia coli. J. Struct. Biol. 146, 123-129.
2003年
Mori, H., Tsukazaki, T., Masui, R., Kuramitsu, S., Yokoyama, S., Johnson, A. E., Kimura, Y., Akiyama, Y. and Ito, K. (2003) Fluorescence resonance energy transfer analysis of protein translocase: SecYE from Thermus thermophilus HB8 forms a constitutive oligomer in membranes. J. Biol. Chem. 278, 14257-14264.
Cairrao, F., Cruz, A., Mori, H. and Arraiano, C. M. (2003) Cold shock induction of RNase R and its role in the maturation of the quality control mediator SsrA/tmRNA.Mol. Microbiol., 50, 1349-1360.
Kanehara, K., Ito, K. and Akiyama, Y (2003) YaeL proteolysis of RseA is controlled by the PDZ domain of YaeL and a Gln-rich region of RseA. EMBO J. 22, 6389-6398.
Satoh, Y., Mori, H. and Ito, K. (2003) Nearest neighbor analysis of the SecYEG complex. II. Identification of a SecY-SecE cytosolic interface. Biochemistry 42, 7442-7447.
Satoh, Y., Matsumoto, G., Mori, H. and Ito, K. (2003) Nearest neighbor analysis of the SecYEG complex. I. Identification of a SecY-SecG interface. Biochemistry 42, 7434-7441.
Akiyama, Y. and Ito, K. (2003) Reconstitution of membrane proteolysis by FtsH. J. Biol. Chem. 278, 18146-18153.
Matsuo, E., Mori, H. and Ito, K. (2003) Interfering mutations provide in vivo evidence that Escherichia coli SecE functions in multimeric states. Mol. Gen. Genomics 268, 808-815.
Shimokawa, N., Mori, H. and Ito, K. (2003) Importance of transmembrane segments in SecY. Mol. Gen. Genomics 269, 180-187.
Mori, H., Akiyama, Y. and Ito, K. (2003) A SecE mutation that modulates SecY-SecE translocase assembly, identified as a specific suppressor against SecY defects. J. Bacteriol. 185, 948-956.
Mori, H. and Ito, K. (2003) Biochemical characterization of a mutationally altered protein translocase: proton-motive force stimulation of the initiation phase of translocation. J. Bacteriol. 185, 405-412.
2002年
Shimohata, N., Chiba, S., Saikawa, N., Ito, K. and Akiyama, Y. (2002) The Cpx stress response system of Escherichia coli senses plasma membrane proteins and controls HtpX, a membrane protease with cytosolic active site. Genes Cells, 7, 653-662.
Mori, H., Shimizu, Y. and Ito, K. (2002) Super active SecY variants that fulfill the essential translocation function with a reduced cellular quantity. J. Biol. Chem. 277, 48550-48557.
Kanehara, K., Ito, K. and Akiyama, Y. (2002) YaeL (EcfE) activates the sE pathway of stress response through a site-2 cleavage of anti-σE, RseA. Genes Dev. 16, 2147-2155.
Chiba, S., Akiyama, Y. and Ito, K. (2002) Membrane protein degradation by FtsH can be initiated from either end. J. Bacteriol. 184, 4775-4782.
Akiyama, Y. (2002) Proton-motive force stimulates the proteolytic activity of FtsH, a membrane-bound ATP-dependent protease in E. coli. Proc. Natl. Acad. Sci. USA 99, 8066-8071.
Chiba, K., Mori, H. and Ito, K. (2002) Roles of the C-terminal end of SecY in protein translocation and viability of Escherichia coli. J. Bacteriol. 184, 2243-2250.
Saikawa, N., Ito, K. and Akiyama, Y. (2002) Identification of glutamic acid 479 as the gluzincin coordinator of zinc in FtsH (HflB). Biochemistry 41, 1861-1868.
2001年
Kanehara, K., Akiyama, Y. and Ito, K. (2001) Characterization of the yaeL gene product and its S2P-protease motifs in Escherichia coli. Gene. 281, 71-79.
Akiyama, Y. and Ito, K. (2001) Roles of the homo-oligomerization and membrane association in the ATPase and the proteolytic activities of FtsH in vitro. Biochemistry 40, 7687-7693.
Kihara, A., Akiyama, Y. and Ito, K. (2001) Revisiting the lysogenization control of bacteriophage l: Identification and characterization of a new host component, HflD. J. Biol. Chem. 276, 13695-13700.
Mori, H. and Ito, K. (2001) An essential amino acid residue in protein translocation channel revealed by targeted random mutagenesis of SecY. Proc. Natl. Acad. Sci. USA. 98, 5128-5133.
2000年
Matsumoto, G., Homma, T., Mori, H. and Ito, K. (2000) A mutation in secY that causes enhanced SecA insertion and impaired late functions in protein translocation. J. Bacteriol., 182, 3377-3382
Nakatogawa, H., Mori, H., Matsumoto, G. and Ito, K. (2000) Characterization of a mutant form of SecA that alleviates a SecY defect at low temperature and shows a synthetic defect with the SecY alteration at high temperature. J. Biochem., 127, 1071 – 1079.
Chiba, S., Akiyama, Y., Mori, H., Matsuo, E. and Ito, K. (2000) Length recognition at the N-terminal tail for the initiation of FtsH-mediated proteolysis. EMBO Reports 1, 47-52.
Akiyama, Y. and Ito, K. (2000) Roles of multimirization and membrane association in the proteolytic functions of FtsH (HflB). EMBO J. 19, 3888-3895.
Tatsuta, T., Joo, D. M., Calendar, R., Akiyama, Y. and Ogura, T. (2000) Evidence for an active role of the DnaK chaperone system in the degradation of s32. FEBS Lett. 478, 271-275.
Nakatogawa, H., Mori, H. and Ito, K. (2000) Two independent mechanisms down-regulate the intrinsic SecA ATPase activity. J. Biol. Chem. 275, 33209-33212.
Matsumoto, G., Nakatogawa, H., Mori, H. and Ito, K. (2000) Genetic dissection of SecA: suppressor mutations against the secY205 translocase defect. Genes Cells 5, 991-1000.
